In this educational webinar, Prof Jason Ong, Director of Melbourne Sexual Health Center, offers a comprehensive overview of sexually transmitted infection (STI) testing, emphasising its critical role in improving sexual health outcomes. The session reviews various current international (WHO, IUSTI) and national (ASHM, BASHH, CDC) guidelines, and provides insights into various testing modalities, including considerations for selecting the most appropriate approach in different clinical settings. Prof Ong also examines the identification of at-risk populations, testing frequency recommendations, as well as follow-up care pathways. Additionally, the webinar explores the benefits and potential harms of asymptomatic testing, providing a balanced perspective to support informed clinical decision-making.